Ernest Šprager

1. HROVAT-ARNEŽ, Katja, ČERNE, Urška, ŠPRAGER, Ernest, GUNČAR, Gregor. Priprava fuzijskega nanotelesa M33-mCherry za detekcijo proteina MLKL. In: KLJUN, Jakob (ed.). Kemijske znanosti 2016. Ljubljana: Fakulteta za kemijo in kemijsko tehnologijo, 2016. Str. 55-56, ilustr. ISBN 978-961-6756-71-6. [COBISS.SI-ID 1537273283]

2. ŠPRAGER, Ernest. Priprava fuzijskega proteina nanotelesa M33 z APEX2 za imunodetekcijo MLKL : diplomsko delo na univerzitetnem študiju Biokemije. Ljubljana: [E. Šprager], 2017. 51 str., ilustr. [COBISS.SI-ID 1537591491]

3. JAZBEC, Vid, ŠPRAGER, Ernest, MEŠKO, Maja, ROMIH, Rok, JERALA, Roman, BENČINA, Mojca. Minimization of gas vesicle cluster. In: KISOVEC, Matic (ed.). Book of abstracts = Knjiga povzetkov. 13th Meeting of the Slovenian Biochemical Society with International Participation = 13. srečanje Slovenskega biokemijskega društva z mednarodno udeležbo, Dobrna, 24 - 27 September 2019. Ljubljana: Slovenian Biochemical Society, 2019. Str. 58. ISBN 978-961-93879-8-6. [COBISS.SI-ID 6727450]

4. ŠPRAGER, Ernest. Produkcija in karakterizacija modificiranih oblik proteinskih plinskih veziklov v E. coli : magistrsko delo. Ljubljana: [E. Šprager], 2020. 60 str., ilustr. Repository of the University of Ljubljana – RUL . [COBISS.SI-ID 19613187]

5. BRATKOVIČ, Tomaž, KRAJNC, Aleksander, ŠPRAGER, Ernest. Activity based profiling of HCPs : vabljeno predavanje na dnevu - Novartisov dan razvoja bioloških zdravil z akademijo, 27. September 2023, Ljubljana. [COBISS.SI-ID 166097155]

6. ŠPRAGER, Ernest, MÖLLER, Jens, LIN, Yuhsien, REISINGER, Veronika, BRATKOVIČ, Tomaž, LUNDER, Mojca, VAŠL, Jožica, KRAJNC, Aleksander. Identification of acyl-protein thioesterase-1 as a polysorbate-degrading host cell protein in a monoclonal antibody formulation using activity-based protein profiling. Journal of pharmaceutical sciences. 2024, vol. 113, iss. 8, str. 2128-2139, graf. prikazi. ISSN 0022-3549. https://www.sciencedirect.com/science/article/pii/S0022354924001850, DOI: 10.1016/j.xphs.2024.05.013. [COBISS.SI-ID 197191939]

7. JAZBEC, Vid, VARDA, Nina, ŠPRAGER, Ernest, MEŠKO, Maja, VIDMAR, Sara, ROMIH, Rok, PODOBNIK, Marjetka, KEŽAR, Andreja, JERALA, Roman, BENČINA, Mojca. Protein gas vesicles of bacillus megaterium as enhancers of ultrasound-induced transcriptional regulation. ACS nano. Jul. 2, 2024, vol. 18, iss. 26, str. 16692–16700. ISSN 1936-086X. https://pubs.acs.org/doi/10.1021/acsnano.4c01498, DiRROS - Digital repository of Slovenian research organizations, Repository of the University of Ljubljana – RUL , DOI: 10.1021/acsnano.4c01498. [COBISS.SI-ID 203392515]

8. LEBAR, Blaž, OREHOVA, Marija Vjačeslavovna, JAPELJ, Boštjan, ŠPRAGER, Ernest, PODLIPEC, Rok, KNAFLIČ, Tilen, URBANČIČ, Iztok, KNEZ, Benjamin, ZIDAR, Mitja, CERAR, Jure, MRAVLJAK, Janez, ŽULA, Aleš, ARČON, Denis, PLAVEC, Janez, PAJK, Stane. A deep dive into spin-labeled polysorbate’s interaction with therapeutic antibody using 2D NMR, EPR and MD simulations. European journal of pharmaceutics and biopharmaceutics. Dec. 2025, vol. 217, art. no. 114892, 1-9 str., ilustr. ISSN 1873-3441. https://www.sciencedirect.com/science/article/pii/S0939641125002693?via%3Dihub, Repository of the University of Ljubljana – RUL , DOI: 10.1016/j.ejpb.2025.114892. [COBISS.SI-ID 255171075]
project: This work was supported by Novartis Pharmaceutical Manufacturing LLC and the Slovenian Research and Innovation Agency (core research funding P1-0208).

9. ŠPRAGER, Ernest, REISINGER, Veronika, SOMMER, Jonas, LEKIĆ, Tinkara, PUCELJ, Nina, KLJUN, Tina, LUNDER, Mojca, VAŠL, Jožica, BRATKOVIČ, Tomaž. Efficient host cell protein clearance : a study of membrane adsorbers and resins in biopharmaceutical processes. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 15 Dec. 2025, vol. 1267, [article no.] 124796, 15 str., ilustr. ISSN 1570-0232. https://www.sciencedirect.com/science/article/pii/S1570023225003502, Repository of the University of Ljubljana – RUL , DOI: 10.1016/j.jchromb.2025.124796. [COBISS.SI-ID 250772739]

10. LEBAR, Blaž, OREHOVA, Marija Vjačeslavovna, JAPELJ, Boštjan, ŠPRAGER, Ernest, PODLIPEC, Rok, KNAFLIČ, Tilen, URBANČIČ, Iztok, KNEZ, Benjamin, ZIDAR, Mitja, CERAR, Jure, MRAVLJAK, Janez, ŽULA, Aleš, ARČON, Denis, PLAVEC, Janez, PAJK, Stane. A multifaceted approach to understanding protein-buffer interactions in biopharmaceuticals. European journal of pharmaceutics and biopharmaceutics. Jan. 2025, vol. 206, [article no.] 114582, str. 1-12, ilustr. ISSN 1873-3441. https://www.sciencedirect.com/science/article/pii/S0939641124004089, DiRROS - Digital repository of Slovenian research organizations, DOI: 10.1016/j.ejpb.2024.114582. [COBISS.SI-ID 219264771]

11. ŠPRAGER, Ernest. Identifikacija hidrolaz gostiteljskih celic in optimizacija njihovega odstranjevanja v proizvodnji terapevtskih protiteles = Identification of host cell hydrolases and optimisation of their removal during therapeutic antibody production : doctoral dissertation. Ljubljana: [E. Šprager], 2026. 1 spletni vir (1 datoteka PDF (144 str.)), ilustr., graf. prikazi. Repository of the University of Ljubljana – RUL . [COBISS.SI-ID 291368963]

12. ŠPRAGER, Ernest (author, corresponding author), KRAJNC, Aleksander (author, corresponding author), LUNDER, Mojca (author, corresponding author), VAŠL, Jožica (author, corresponding author), BRATKOVIČ, Tomaž (author, corresponding author). Persistence and Fab-specific interaction of acyl-protein thioesterase-1 with monoclonal antibodies during downstream processing. Protein expression and purification. Jul. 2026, vol. 241, [article no.] 106920, str. 1-7, ilustr. ISSN 1046-5928. https://www.sciencedirect.com/science/article/pii/S1046592826000434, Repository of the University of Ljubljana – RUL , DOI: 10.1016/j.pep.2026.106920. [COBISS.SI-ID 272547075]
project: This work was supported by Novartis Pharmaceutical Manufacturing LLC and the Slovenian Research and Innovation Agency (core research funding P1-0420).